Research Postdoctoral Fellow - Structural Immunology and Protein Engineering Sgourakis Lab

Responsibilities will include: Recombinant protein expression, in vitro refolding, and biophysical characterization (DSF, SEC, ITC, SPR, fluorescence polarization) of MHC-I/MR1 constructs Solution NMR sample preparation, isotopic labeling, and data acquisition (backbone and methyl-based experiments)Collaborative structural and mechanistic studies of chaperone-mediated ligand exchange Manuscript preparation and contribution to grant-funded research objectives

What you will do: Conduct independent scholarly research and develop new methods and protocols Participate in project planning, recording and evaluation of data Acquire technical, lab management and grant writing skills Participate in seminars, lectures, poster sessions and presentation at national meetings Supervise junior lab members Assist with other research projects

Qualifications: Ph. D. in Biochemistry, Biophysics, Structural Biology, Immunology, or a related field Hands-on experience with recombinant protein expression/purification and biophysical assays preferred Experience with solution NMR (sample preparation and/or data analysis) strongly preferred Strong record of scientific writing and communication, as evidenced by peer-reviewed publications Ability to work both independently and collaboratively in a multidisciplinary research environment Environment: The lab is based in the Center for Computational and Genomic Medicine at CHOP, with primary faculty appointment in the Department of Biochemistry and Biophysics at the University of Pennsylvania. Trainees have access to state-of-the-art NMR (600/800 MHz), biophysics, and cryo-EM facilities, and close collaborations across CHOP and Penn in structural biology, immunology, and cell and gene therapy. Selected Publications: McShan A.C., Devlin C., Papadaki G.F., Sun Y., Green A.I., Morozov G.I., Burslem G.M., Procko E., Sgourakis N.G. TAPBPR employs a ligand-independent docking mechanism to chaperone MR1 molecules. Nature Chemical Biology 18(8):859–868 (2022).Rotsides P., Shinde O., Danon J.N., Sgourakis N.G. A miniaturized MR1 metabolite display system with native-like protein features. eLife 2026;15:RP111511.

Skills and Abilities: Ability to wear a respirator device To carry out its mission, CHOP is committed to supporting the health of our patients, families, workforce, and global community. As a condition of employment, CHOP employees who work in patient care buildings or who have patient facing responsibilities must receive an annual influenza vaccine. Salary ranges are shown for full-time jobs. If you're working part-time, your pay will be adjusted accordingly. For more information regarding CHOP's Compensation and Benefits, click here.