About the role

Senior Associate Scientist PharmaEssentia Corporation is seeking a Senior Associate Scientist to join the Cell Therapy team and contribute to the development of novel immunotherapies for immunology and oncology. The successful candidate will be responsible for mRNA-based CAR T cell engineering and characterization. The successful candidate will be a highly motivated bench scientist and will be responsible for immuno-assay execution for discovery projects and technology evaluations. They will generate data to support key decisions for project teams and actively participate in the interpretation of data and design of experiments. They will have the opportunity to work independently and collaboratively to advance portfolio programs in a dynamic and fast-paced environment. PharmaEssentia Corporation is a rapidly growing biopharmaceutical innovator. We are leveraging deep expertise and proven scientific principles to deliver effective new biologics for challenging diseases in the areas of hematology and oncology, with one approved product and a diversifying pipeline. We believe in the potential to improve both health and quality of life for patients with limited options today through the combination of rigorous research and innovative thinking. Summary of Key Responsibilities: Perform primary immune cell isolation and culture, as well as cancer cell line culture. Perform mRNA-based CAR T cell engineering using electroporation and tLNPLead functional characterization of CAR T cells with immunoassays Data analysis and thorough documentation of work in the Company's ELN system Present results at team meetings, in other group settings, and at external scientific conferences Work cross-functionally as part of internal multi-disciplinary project teams Maintaining a safe and clean laboratory environment following all precautions required for a BSL2/BSL2+ setting.
Essential Qualifications:
  • Senior Associate Scientist: B.S./M.S. in immunology, biology, oncology or related discipline with BS 6+ years or MS 4+ years of relevant industry experience
  • Strong hands-on experience with primary human immune cell and cancer cell line culture, T cell transfection via mRNA electroporation, cell immunophenotyping using multicolor flow cytometry, cytotoxicity assays, multiplex ELISA, live-cell analysis using incucyte system
  • Proficiency in operating instruments like BD Fortessa flow cytometer, Lonza 4D-Nucleofector, Meso Scale Discovery plate reader, xCELLigence RTCAWorking knowledge of CAR T cell therapy discovery
  • Ability to follow defined procedures and protocols, and strong attention to detail
  • Proficiency with data analysis software such as FlowJo, GraphPad Prism, etc
  • Willingness to learn new research methods and a desire for continued learning and development
  • Excellent organization and record-keeping skills
  • Collaborative, flexible, and team-oriented mindset, with the ability to contribute effectively within a dynamic research environment
Preferred Qualifications:
  • Direct experiences with mRNA/tLNP CAR T engineering and characterization
  • Knowledge of building high-throughput T cell engineering and characterization workflow
  • Track record of assay development and optimization in an in vitro pharmacology setting
Knowledge of molecular biology techniques, including mRNA IVT, real-time PCR, and ddPCRExperience in immunophenotyping tumor-infiltrating T cells in CDX/PDX mouse modelEEO Statement: At PharmaEssentia Innovation Research Center (PIRC), we are committed to providing an environment of mutual respect where equal employment opportunities are available to all applicants without regard to race, color, religion, sex, national origin, age, physical and mental disability, marital status, sexual orientation, gender identity, gender expression, genetic information (including characteristics and testing), military and veteran status, and any other characteristic protected by applicable law. PIRC believes that diversity and inclusion among our team are critical to our success as a global company, and we seek to recruit, develop and retain the most talented people from a diverse candidate pool. At PharmaEssentia, our goal is to treat as many people with cancer as possible. That means challenging the status quo with better science that leads to better lives. By joining our team, you will not only expand your own possibilities, but you will contribute to expanding options for people with cancer.

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JOB OVERVIEW

Experience level

Senior

Location

Bedford, MA

Occupation

Molecular and Cellular Biologists

Industry

Research and Development in Biotechnology (except Nanobiotechnology)

Posted

13 days ago

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